The points in dSTORM acquisitions have the properties shown in the right-hand panel. Sliders can be used to filter out points by choosing ranges for these properties.
The checkbox in the top left corner of each slider is used to determine whether that property will be used to filter the points. The number of properties displayed depends on those present in the data.
The localisation algorithm runs a statistical fitting procedure on each localisation, producing a number of properties. Those available through the filtering tool are summarised here:
If a dataset has multiple channels, then the set of channels to be filtered can be selected in the visualisation panel as shown below where two channels have been selected (highlighted borders). Individual channels can also be selected separately to apply different filters to each.
Once the filters have been selected, the current parameter settings can then be saved by clicking on the save button on the top right corner of the right hand side panel. These settings can be saved to the cloud (on CODI) or on the local machine. If saved on the local machine, a JSON file is downloaded showing the parameters that have been changed and the corresponding ranges. An example of a downloaded file is shown below. This same file can be loaded into the CODI filtering tool using the load button and that will automatically apply the filters to the image.